IPTG 5 g
- N° CAS : 367-93-1
Reference :
I1401.0005
Marque :
Isopropyl-ß-D-thiogalactoside. IPTG is a chemical analogue of galactose that cannot be cleaved by ß-galactosidase. Functioning as an analogue. IPTG binds and inhibits the powerful lac repressor. strongly inducing the production of ß-galactosidase..IPTG is used in conjunction with X-Gal for detection of lac+ colonies or cells in a colorimetric assay. in order to distinguish recombinants (white) from non recombinants (blue) in cloning strategies using vectors like Lamb-da-11. M13m-p18 and 19. pUC18 and 19. pUR222 containing the lacZ gene. For more detailed information see X-Gal.